FIREBAL Web

Input files

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Image TIFF — click or drop

ZYX / CZYX / TZCYX · multi-channel · time-series · 2D/3D

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PSF TIFF — click or drop (optional)

Leave empty to generate PSF server-side from optical parameters

Stack layout

Axis orderfastest last · Fiji default: TZCYX

Channels

All channels Single:

Timepoints

All timepoints or T =

Number of channelsfor per-channel wavelength table

PSF generation

Used when no PSF file is uploaded. PSF grid size is set automatically to match the tile size.

Modality

Voxel size (µm)

dZ dY dX

Emission channel

PSF is computed with the symmetric scalar model.

Wavelength (µm)

NA

n medium

Spherical aberrationZ(4,0) coefficient

Presets

Tiling

Tile size is computed automatically from available GPU memory.

Enable tilingfor volumes larger than VRAM Use tiled processing

Poisson pad Z / Y / X

Z Y X

Richardson-Lucy parameters

Iterations

Wiener param Δ

OTF threshold

Background α

Wiener adapt γ0 = fixed

Upsample factor

File

Filename

Bit depth

Depth

uint16 (0–65535) uint8 (0–255) float32 (raw)

Integer output is min-max scaled after negative clipping. float32 is raw.
Result saved as TZCYX hyperstack — Fiji-compatible.
Voxel size is taken from PSF voxel size settings.

Select a job from the queue to view its status and download the result.